In addition, a significant positive correlation was found between Ct and time-to-positivity with Lucira (R = 0.8612, p < 0.0001). All samples with a positive RT-PCR result with a threshold cycle (Ct) > 36, yielded a negative Lucira result. Among the 72 symptomatic participants, 67 (93%) tested positive by Lucira. Out of 90 participants who had a positive RT-PCR result, 82 (91.1%) tested positive by Lucira. All negative RT-PCR results were paralleled by a negative Lucira result. Each sample was also subjected to RT-PCR. This study evaluated the clinical sensitivity and specificity of Lucira in identifying the virus in 190 nasopharyngeal samples collected between January and October 2021. One such test is the Lucira™ Check It COVID-19 Test kit ("Lucira") (Lucira Health, Inc., Emeryville, CA, USA), which utilizes real-time loop-mediated isothermal amplification technology, developed for at-home use. The need for the early identification of SARS-CoV-2 has let to a quest for reliable tests that meet the standards of polymerase chain reaction (PCR) tests, on the one hand, and are low-cost, easy-to-use, and fast, on the other hand.
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